Application
A. Western blot analysis on HeLa cell lysates using anti-Histone H2B Acetyl (K15) RabMAb (Cat. #AJ1356b), 1:2000 dilution. Cells were either (A) untreated (B) treated with TSA.Application
B. Immunohistochemical staining of paraffin-embedded human breast carcinoma using Anti-Histone H2B Acetyl (K15) RabMAb (Cat. #AJ1356b).| Product Name | Histone-H2B Antibody Acetyl (K15) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | Acetyl K15 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP955Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | HIST2H2BF Histone H2B type 2-F |
|---|---|
| Molecular Weight(MW) | 13920 Da |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling |
| Tissue Specificity | A synthetic acetylated peptide corresponding to residues surrounding Lysine 15 of Histone H2B was used as an immunogen. The antibody only detects Histone H2B acetylated on Lysine 15. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 440689 |
|---|
Application
A. Western blot analysis on HeLa cell lysates using anti-Histone H2B Acetyl (K15) RabMAb (Cat. #AJ1356b), 1:2000 dilution. Cells were either (A) untreated (B) treated with TSA.
Application
B. Immunohistochemical staining of paraffin-embedded human breast carcinoma using Anti-Histone H2B Acetyl (K15) RabMAb (Cat. #AJ1356b).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
