Application
A. Western blot analysis on A431 cell lysate using anti-Histone H2B Acetyl (K5) RabMAb (Cat. #AJ1356d), 1:50,000 dilution. Cells were (1) untreated or (2) treated with TSA.Application
B. Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma using anti-Histone H2B acetyl- K5 RabMAb (Cat. #AJ1356d).Application
C. Immunofluorescent staining of A431 cells using anti-Histone H2B acetyl-K5 Rab MAb (Cat. #AJ1356d).| Product Name | Histone-H2B Antibody Acetyl (K5) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | Acetyl K5 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP857Y |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:50000~ IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | HIST2H2BF Histone H2B type 2-F |
|---|---|
| Molecular Weight(MW) | 13920 Da |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling |
| Tissue Specificity | A synthetic acetylated peptide corresponding to residues surrounding Lys 5 of Histone H2B was used as an immunogen. The antibody only detects Histone H2B acetylated on Lys 5. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 440689 |
|---|
Application
A. Western blot analysis on A431 cell lysate using anti-Histone H2B Acetyl (K5) RabMAb (Cat. #AJ1356d), 1:50,000 dilution. Cells were (1) untreated or (2) treated with TSA.
Application
B. Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma using anti-Histone H2B acetyl- K5 RabMAb (Cat. #AJ1356d).
Application
C. Immunofluorescent staining of A431 cells using anti-Histone H2B acetyl-K5 Rab MAb (Cat. #AJ1356d).| Application Notes | WB:1:50000~ IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
