Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-Histone H3 (pT3) RabMAb (Cat. #AJ1353e). Cells were either (A) untreated (B) treated with FBS + Calyculin A.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Histone H3 RabMAb (Cat. #AJ1353e).| Product Name | Histone H3 Antibody Phospho (pT3) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-T3 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1702Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:10000~50000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | H3FA |
|---|---|
| Alternative Names | HIST1H3J H3FJ Histone H3.1 H3/a H3/b H3/c H3/d H3/f H3/h H3/i H3/j H3/k H3/l |
| Molecular Weight(MW) | 15404 Da |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling |
| Tissue Specificity | A phospho specific peptide corresponding to residues surrounding Threonine 3 of human Histone H3 was used as an immunogen. This antibody detects Histone H3 phosphorylated at Theronine 3. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 8968 |
|---|
Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-Histone H3 (pT3) RabMAb (Cat. #AJ1353e). Cells were either (A) untreated (B) treated with FBS + Calyculin A.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Histone H3 RabMAb (Cat. #AJ1353e).| Application Notes | WB:1:10000~50000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
