Application
A. Western blot analysis on HeLa cell lysates using anti- Histone H3.3 Phospho (pS31) RabMAb (Cat. #AJ1354a). Cells were either (A) untreated (B) treated with Calyculin.Application
B. Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using anti-Histone H3.3 RabMAb (Cat. #AJ1354a).Application
C. Immunofluorescent staining of HeLa cells using anti-Histone H3.3 RabMAb (Cat. #AJ1354a).| Product Name | Histone H3.3 Antibody Phospho (pS31) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S31 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR1873 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:1000~5000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | H3.3A, H3F3 |
|---|---|
| Molecular Weight(MW) | 15328 Da |
| Function | Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling |
| Tissue Specificity | A synthetic peptide corresponding to residues surrounding Serine 31 of human Histone H3.3 was used as an immunogen. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 3021 |
|---|
Application
A. Western blot analysis on HeLa cell lysates using anti- Histone H3.3 Phospho (pS31) RabMAb (Cat. #AJ1354a). Cells were either (A) untreated (B) treated with Calyculin.
Application
B. Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using anti-Histone H3.3 RabMAb (Cat. #AJ1354a).
Application
C. Immunofluorescent staining of HeLa cells using anti-Histone H3.3 RabMAb (Cat. #AJ1354a).| Application Notes | WB:1:1000~5000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
