Application
A. Western blot nalysis on HeLa cell lysates using anti-Phospho-Histone H3.3 (pS28 & S31) RabMAb (Cat. #AJ1358a), 1:5000 dilution. Cells were either (A) untreated (B) treated with Hela+FBS +calyculin A| Product Name | Histone-H3.3 Antibody Phospho-pS28&31 |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S28&31 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1874Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | H3.3A, H3F3 |
|---|---|
| Alternative Names | H3f3b H3.3b Histone H3.3 |
| Molecular Weight(MW) | 15328 Da |
| Function | Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling |
| Tissue Specificity | A synthetic phospho-peptide corresponding to residues surrounding serine 28 and serine 31 of human Histone H3.3 protein. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 3021 |
|---|
Application
A. Western blot nalysis on HeLa cell lysates using anti-Phospho-Histone H3.3 (pS28 & S31) RabMAb (Cat. #AJ1358a), 1:5000 dilution. Cells were either (A) untreated (B) treated with Hela+FBS +calyculin A| Application Notes | WB:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
