Application
A. Western blot analysis on C6 cell lysate using anti-Histone H3T Acetyl (K27) RabMAb (Cat. #AJ1359a), 1:500 dilution. Cells were either (1) untreated or (2) treated with TSA.Application
B. Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using anti-H3T Acetyl-K27 RabMAb. (Cat. #AJ1359a).Application
C. Immunofluorescent staining of A431 cells using anti-Histone H3T Acetyl-K27 RabMAb. (Cat. #AJ1359a).| Product Name | Histone-H3T Antibody Acetyl (K27) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | Acetyl K27 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP865Y |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | H3FA |
|---|---|
| Alternative Names | HIST2H2BF Histone H2B type 2-F |
| Molecular Weight(MW) | 15404 Da |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling |
| Tissue Specificity | A synthetic acetylated peptide corresponding to residues surrounding Lys 27 of Histone H3T was used as immunogen. The antibody only detects Histone H3T acetylated on Lysine 27. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 8968 |
|---|
Application
A. Western blot analysis on C6 cell lysate using anti-Histone H3T Acetyl (K27) RabMAb (Cat. #AJ1359a), 1:500 dilution. Cells were either (1) untreated or (2) treated with TSA.
Application
B. Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using anti-H3T Acetyl-K27 RabMAb. (Cat. #AJ1359a).
Application
C. Immunofluorescent staining of A431 cells using anti-Histone H3T Acetyl-K27 RabMAb. (Cat. #AJ1359a).| Application Notes | WB:1:500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
