Application
A. Western blot analysis on A.) C6 cell lysate and B.) C6 cell + TSA lysate using anti-Histone H4, Acetyl-K8 RabMAb (Cat. #AJ1360b), dilution 1:10,000.Application
B. Immunofluorescent staining of Hela cells using anti-Histone H4 RabMAb. (Cat. #AJ1360b).Application
C. Immunohistochemical staining of paraffin-embedded human cervical carcinoma using anti-Histone H4 RabMAb (Cat. #AJ1360b).| Product Name | Histone-H4 Antibody Acetyl (K8) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | Acetyl K8 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1002Y |
| Host Species | Rabbit |
| Tested Applications | WBIFIHC |
| WB:1:10000~50000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | H4/A, H4FA |
|---|---|
| Alternative Names | HIST4H4 H4/O H4FO Histone H4 |
| Molecular Weight(MW) | 11367 Da |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling |
| Tissue Specificity | A synthetic peptide corresponding to residues near the N-terminus of human Histone H4 was used as an immunogen. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 8370 |
|---|
Application
A. Western blot analysis on A.) C6 cell lysate and B.) C6 cell + TSA lysate using anti-Histone H4, Acetyl-K8 RabMAb (Cat. #AJ1360b), dilution 1:10,000.
Application
B. Immunofluorescent staining of Hela cells using anti-Histone H4 RabMAb. (Cat. #AJ1360b).
Application
C. Immunohistochemical staining of paraffin-embedded human cervical carcinoma using anti-Histone H4 RabMAb (Cat. #AJ1360b).| Application Notes | WB:1:10000~50000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
