Application
HJURP Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HJURP antibody detected the HJURP protein (arrow).Application
HJURP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HJURP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
HJURP Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | HJURP Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FAKTS, FLEG1, URLC9 |
|---|---|
| Alternative Names | HJURP FAKTS FLEG1 URLC9 Holliday junction recognition protein 14-3-3-associated AKT substrate Fetal liver-expressing gene 1 protein Up-regulated in lung cancer 9 |
| Molecular Weight(MW) | 83539 Da |
| Function | Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds Holliday junctions |
| Tissue Specificity | According to PubMed:17256767, highly expressed in the thymus with lower levels in the placenta, small intestine, liver, skeletal muscle, and colon. According to PubMed:17823411, highly expressed in testis, and at a relatively lower level in thymus and bone marrow. Significantly overexpressed in many lung cancer samples, compared with normal lung |
| Cellular Localization | Nucleus, nucleolus. Chromosome, centromere. Note=Localizes in centromeres during late telophase and early G1, when CENPA nucleosomes are assembled. Localizes to nucleolus during S phase, nucleolus site being often related to storage |
| Entrez Gene | 55355 |
|---|---|
| Nucleotide Accession | NP_060880.3 |
Application
HJURP Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HJURP antibody detected the HJURP protein (arrow).
Application
HJURP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HJURP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
HJURP Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
