Application
The anti-HK1 Pab is used in Western blot to detect HK1 in mouse skeletal muscle tissue lysate.Application
Western blot analysis of HK1 (arrow) using HK1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HK1 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
HK1 Antibody (L93) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the HK1 antibody detected the HK1 protein (arrow).| Product Name | HK1 (Hexokinase) Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | HK1 Hexokinase-1 Brain form hexokinase Hexokinase type I |
|---|---|
| Molecular Weight(MW) | 102486 Da |
| Tissue Specificity | Isoform 2 is erythrocyte specific. Isoform 3 and isoform 4 are testis-specific |
| Cellular Localization | Mitochondrion outer membrane. Note=Its hydrophobic N-terminal sequence may be involved in membrane binding |
| Entrez Gene | 3098 |
|---|
Application
The anti-HK1 Pab is used in Western blot to detect HK1 in mouse skeletal muscle tissue lysate.
Application
Western blot analysis of HK1 (arrow) using HK1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HK1 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
HK1 Antibody (L93) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the HK1 antibody detected the HK1 protein (arrow).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
