Application
Western blot analysis of HLA-B Antibody (N-term) in A2058 cell line lysates (35ug/lane). HLA-B (arrow) was detected using the purified Pab.Application
Western blot analysis of HLA-B Antibody (N-term) in A375 cell line lysates (35ug/lane). HLA-B (arrow) was detected using the purified Pab.Application
Western blot analysis of HLA-B (arrow) using rabbit polyclonal HLA-B Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-B gene.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HLA-B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
HLA-B Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | HLA-B Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HLAB |
|---|---|
| Alternative Names | HLA-B HLAB HLA class I histocompatibility antigen B-27 alpha chain MHC class I antigen B*27 |
| Molecular Weight(MW) | 40428 Da |
| Function | Involved in the presentation of foreign antigens to the immune system |
| Tissue Specificity | This HLA-B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69~99 amino acids from the N-terminal region of human HLA-B. |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
Western blot analysis of HLA-B Antibody (N-term) in A2058 cell line lysates (35ug/lane). HLA-B (arrow) was detected using the purified Pab.
Application
Western blot analysis of HLA-B Antibody (N-term) in A375 cell line lysates (35ug/lane). HLA-B (arrow) was detected using the purified Pab.
Application
Western blot analysis of HLA-B (arrow) using rabbit polyclonal HLA-B Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-B gene.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HLA-B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
HLA-B Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
