Application
A. Western blot analysis on (A) SKBR-3, (B) Hela and (C) HepG2 cell lysates using anti-HMGB1 RabMAb (Cat. #AJ1364a), dilution 1:50,000.Application
B. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HMGB1 RabMAb (Cat. #AJ1364a).| Product Name | HMGB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HMGB1, HMG1, High mobility group protein B1, High mobility group protein 1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3507 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:10000~50000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HMG1 |
|---|---|
| Alternative Names | HMGB1 HMG1 High mobility group protein B1 High mobility group protein 1 |
| Molecular Weight(MW) | 24894 Da |
| Function | DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells (By similarity) |
| Tissue Specificity | A synthetic peptide corresponding to residues in human HMGB1 was used as an immunogen. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 3146 |
|---|
Application
A. Western blot analysis on (A) SKBR-3, (B) Hela and (C) HepG2 cell lysates using anti-HMGB1 RabMAb (Cat. #AJ1364a), dilution 1:50,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HMGB1 RabMAb (Cat. #AJ1364a).| Application Notes | WB:1:10000~50000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
