Application
HMGB1 Antibody (C-term) (Cat. #AM1991a) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the HMGB1 antibody detected the HMGB1 protein (arrow).Application
HMGB1 Antibody (C-term) (Ascites) (AM1991a)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HMGB1 Antibody (C-term) (Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | HMGB1 Antibody (C-term) (Ascites) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | 393CT23.1.4 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500~1600 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HMG1 |
|---|---|
| Alternative Names | HMGB1 HMG1 High mobility group protein B1 High mobility group protein 1 |
| Molecular Weight(MW) | 24894 Da |
| Function | DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells (By similarity) |
| Tissue Specificity | This HMGB1 Monoclonal antibody is generated from mouses immunized with a KLH conjugated synthetic peptide selected from the C-term region of human HMGB1. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 3146 |
|---|---|
| Nucleotide Accession | NP_002119.1 |
Application
HMGB1 Antibody (C-term) (Cat. #AM1991a) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the HMGB1 antibody detected the HMGB1 protein (arrow).
Application
HMGB1 Antibody (C-term) (Ascites) (AM1991a)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HMGB1 Antibody (C-term) (Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:500~1600 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
