Application
Western blot analysis of HMGCS2 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). HMGCS2 (arrow) was detected using the purified Pab.Application
HMGCS2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HMGCS2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
HMGCS2 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | HMGCS2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | HMGCS2 Hydroxymethylglutaryl-CoA synthase mitochondrial 3-hydroxy-3-methylglutaryl coenzyme A synthase |
|---|---|
| Molecular Weight(MW) | 56635 Da |
| Function | This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase |
| Tissue Specificity | High expression in liver and colon. Low expression in testis, heart, skeletal muscle and kidney |
| Cellular Localization | Mitochondrion. |
| Entrez Gene | 3158 |
|---|
Application
Western blot analysis of HMGCS2 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). HMGCS2 (arrow) was detected using the purified Pab.
Application
HMGCS2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HMGCS2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
HMGCS2 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
