Application
Western blot analysis of HMX3 Antibody (Center) in HepG2 cell line lysates (35ug/lane). HMX3 (arrow) was detected using the purified Pab.Application
Western blot analysis of HMX3 Antibody (Center) in NIH-3T3 cell line lysates (35ug/lane). HMX3 (arrow) was detected using the purified Pab.Application
HMX3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HMX3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
HMX3 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | HMX3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NKX-5.1, NKX5-1 |
|---|---|
| Alternative Names | HMX3 NKX-5.1 NKX5-1 Homeobox protein HMX3 Homeobox protein H6 family member 3 Homeobox protein Nkx-5.1 |
| Molecular Weight(MW) | 37825 Da |
| Function | Transcription factor involved in specification of neuronal cell types and which is required for inner ear and hypothalamus development. Binds to the 5'-CAAGTG-3' core sequence Controls semicircular canal formation in the inner ear. Also required for hypothalamic/pituitary axis of the CNS (By similarity) |
| Tissue Specificity | This HMX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153~182 amino acids from the Center region of human HMX3. |
| Cellular Localization | Nucleus (By similarity). |
Application
Western blot analysis of HMX3 Antibody (Center) in HepG2 cell line lysates (35ug/lane). HMX3 (arrow) was detected using the purified Pab.
Application
Western blot analysis of HMX3 Antibody (Center) in NIH-3T3 cell line lysates (35ug/lane). HMX3 (arrow) was detected using the purified Pab.
Application
HMX3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HMX3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
HMX3 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
