Application
Confocal immunofluorescent analysis of HNMT Antibody (N-term)(Cat#AP1442a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of HNMT Antibody (N-term) (Cat# AP1442a) in Hela cell line lysates (35ug/lane). HNMT(arrow) was detected using the purified polyclonal antibody.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HNMT antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | HNMT Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HNMT; Histamine N-methyltransferase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWBIHC |
| IF:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | HNMT Histamine N-methyltransferase |
|---|---|
| Molecular Weight(MW) | 33295 Da |
| Function | Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine |
| Tissue Specificity | This HNMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36~66 amino acids from the N-terminal region of human HNMT. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 3176 |
|---|
Application
Confocal immunofluorescent analysis of HNMT Antibody (N-term)(Cat#AP1442a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of HNMT Antibody (N-term) (Cat# AP1442a) in Hela cell line lysates (35ug/lane). HNMT(arrow) was detected using the purified polyclonal antibody.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HNMT antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | IF:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
