Application
A.Western blot analysis on (A) HeLa, (B) 293T, (C) MCF-7 and (C) human cerebellum lysates using anti-hnRNP RabMAb (Cat. #AJ1367a), dilution 1:100,000.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-hnRNP C1/C2 RabMAb (Cat. #AJ1367a).| Product Name | hnRNP C1/C2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HNRNPC, HNRPC, Heterogeneous nuclear ribonucleoproteins C1/C2 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP3034Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50000~100000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HNRPC |
|---|---|
| Alternative Names | HNRNPC HNRPC Heterogeneous nuclear ribonucleoproteins C1/C2 |
| Molecular Weight(MW) | 33670 Da |
| Function | Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Single HNRNPC tetramers bind 230-240 nucleotides Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules |
| Tissue Specificity | A synthetic peptide corresponding to residues near the N-terminus of human hnRNP C1/C2 was used as an immunogen. |
| Cellular Localization | Nucleus. Note=Component of ribonucleosomes. |
| Entrez Gene | 3183 |
|---|
Application
A.Western blot analysis on (A) HeLa, (B) 293T, (C) MCF-7 and (C) human cerebellum lysates using anti-hnRNP RabMAb (Cat. #AJ1367a), dilution 1:100,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-hnRNP C1/C2 RabMAb (Cat. #AJ1367a).| Application Notes | WB:1:50000~100000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
