Application
A. Western blot analysis on Jurkat cell lysate using anti-hn RNP RabMAb (Cat. #AJ1368a), dilution 1:100,000.Application
B. Immunohistochemical staining of paraffin-embedded human breast cancer tissue using anti-hnRNP RabMAb (Cat. #AJ1368a).Application
C. Fluorescent immunostaining of HeLa cells using anti-hnRNP RabMAb. (Cat. #AJ1368a).| Product Name | hnRNP K Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP943Y |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HNRPK |
|---|---|
| Alternative Names | HNRNPK HNRPK Heterogeneous nuclear ribonucleoprotein K Transformation up-regulated nuclear protein |
| Molecular Weight(MW) | 50976 Da |
| Function | One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single- stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest |
| Tissue Specificity | A synthetic peptide corresponding to residues near the N-terminus of human hnRNP was used as an immunogen. This antibody detects both phosphorylated and unphosphorylated hnRNP K. |
| Cellular Localization | Cytoplasm. Nucleus, nucleoplasm. Note=Recruited to p53/TP53-responsive promoters, in the presence of functional p53/TP53. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear |
| Entrez Gene | 3190 |
|---|
Application
A. Western blot analysis on Jurkat cell lysate using anti-hn RNP RabMAb (Cat. #AJ1368a), dilution 1:100,000.
Application
B. Immunohistochemical staining of paraffin-embedded human breast cancer tissue using anti-hnRNP RabMAb (Cat. #AJ1368a).
Application
C. Fluorescent immunostaining of HeLa cells using anti-hnRNP RabMAb. (Cat. #AJ1368a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
