Application
Western blot analysis of anti-HNRPK Antibody (N-term) in 293 cell line lysates (35ug/lane). HNRPK(arrow) was detected using the purified Pab.Application
Western blot analysis of anti-HNRPK Antibody (N-term) in mouse brain tissue lysates (35ug/lane). HNRPK(arrow) was detected using the purified Pab.| Product Name | HNRPK Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HNRNPK; HNRPK; Heterogeneous nuclear ribonucleoprotein K; Transformation up-regulated nuclear protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HNRPK |
|---|---|
| Alternative Names | HNRNPK HNRPK Heterogeneous nuclear ribonucleoprotein K Transformation up-regulated nuclear protein |
| Molecular Weight(MW) | 50976 Da |
| Function | One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single- stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest |
| Tissue Specificity | This HNRPK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human HNRPK. |
| Cellular Localization | Cytoplasm. Nucleus, nucleoplasm. Note=Recruited to p53/TP53-responsive promoters, in the presence of functional p53/TP53. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear |
| Entrez Gene | 3190 |
|---|
Application
Western blot analysis of anti-HNRPK Antibody (N-term) in 293 cell line lysates (35ug/lane). HNRPK(arrow) was detected using the purified Pab.
Application
Western blot analysis of anti-HNRPK Antibody (N-term) in mouse brain tissue lysates (35ug/lane). HNRPK(arrow) was detected using the purified Pab.| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
