Application
Western blot analysis of HNRPQ Antibody (Center) in T47D cell line lysates (35ug/lane). HNRPQ (arrow) was detected using the purified Pab.Application
HNRPQ Antibody (Center) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HNRPQ Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
HNRPQ Antibody (Center) flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | HNRPQ Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HNRPQ, NSAP1 |
|---|---|
| Alternative Names | SYNCRIP HNRPQ NSAP1 Heterogeneous nuclear ribonucleoprotein Q Glycine- and tyrosine-rich RNA-binding protein NS1-associated protein 1 Synaptotagmin-binding cytoplasmic RNA-interacting protein |
| Molecular Weight(MW) | 69603 Da |
| Function | Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD- mediated complex that promotes MYC mRNA stability. Isoform 1, isoform 2 and isoform 3 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 3 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma- induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation; seems not to be essential for GAIT complex function |
| Tissue Specificity | Ubiquitously expressed. Detected in heart, brain, pancreas, placenta, spleen, lung, liver, skeletal muscle, kidney, thymus, prostate, uterus, small intestine, colon, peripheral blood and testis |
| Cellular Localization | Cytoplasm. Microsome (By similarity). Endoplasmic reticulum (By similarity). Nucleus (By similarity) Note=The tyrosine phosphorylated form bound to RNA is found in microsomes (By similarity). Localized in cytoplasmic mRNP granules containing untranslated mRNAs Isoform 2: Nucleus, nucleoplasm (By similarity). Note=Expressed predominantly in the nucleoplasm (By similarity) |
| Entrez Gene | 10492 |
|---|
Application
Western blot analysis of HNRPQ Antibody (Center) in T47D cell line lysates (35ug/lane). HNRPQ (arrow) was detected using the purified Pab.
Application
HNRPQ Antibody (Center) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HNRPQ Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
HNRPQ Antibody (Center) flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
