Application
Western blot analysis of HOMER3 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane).HOMER3 (arrow) was detected using the purified Pab.Application
Western blot analysis of HOMER3 Antibody (Center) in mouse NIH-3T3 cell line lysates (35ug/lane).HOMER3 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue with HOMER3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of SK-Br-3 cells using HOMER3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of HOMER3 (arrow) using rabbit polyclonal HOMER3 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HOMER3 gene.| Product Name | HOMER3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HOMER3; Homer protein homolog 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | HOMER3 Homer protein homolog 3 |
|---|---|
| Molecular Weight(MW) | 39836 Da |
| Function | Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses |
| Tissue Specificity | This HOMER3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287~317 amino acids from the Center region of human HOMER3. |
| Cellular Localization | Cytoplasm (By similarity). Cell junction, synapse, postsynaptic cell membrane, postsynaptic density (By similarity). Cell junction, synapse (By similarity) Note=Postsynaptic density of neuronal cells (By similarity) |
| Entrez Gene | 9454 |
|---|
Application
Western blot analysis of HOMER3 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane).HOMER3 (arrow) was detected using the purified Pab.
Application
Western blot analysis of HOMER3 Antibody (Center) in mouse NIH-3T3 cell line lysates (35ug/lane).HOMER3 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue with HOMER3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of SK-Br-3 cells using HOMER3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of HOMER3 (arrow) using rabbit polyclonal HOMER3 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HOMER3 gene.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
