Application
HOMEZ Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the HOMEZ antibody detected the HOMEZ protein (arrow).Application
HOMEZ Antibody (N-term) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Fluorescent confocal image of A431 cell stained with HOMEZ Antibody (N-term)(Cat#AP10772a).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOMEZ primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HOMEZ immunoreactivity is localized to Nucleus significantly.| Product Name | HOMEZ Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HOMEZ; KIAA1443; Homeobox and leucine zipper protein Homez; Homeodomain leucine zipper-containing factor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIF |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1443 |
|---|---|
| Alternative Names | HOMEZ KIAA1443 Homeobox and leucine zipper protein Homez Homeodomain leucine zipper-containing factor |
| Molecular Weight(MW) | 61240 Da |
| Function | May function as a transcriptional regulator. |
| Tissue Specificity | Ubiquitous. Strongly expressed in adult testis and kidney as well as fetal lung and kidney |
| Cellular Localization | Nucleus. |
| Entrez Gene | 57594 |
|---|---|
| Nucleotide Accession | NP_065885.2 |
Application
HOMEZ Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the HOMEZ antibody detected the HOMEZ protein (arrow).
Application
HOMEZ Antibody (N-term) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Fluorescent confocal image of A431 cell stained with HOMEZ Antibody (N-term)(Cat#AP10772a).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOMEZ primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HOMEZ immunoreactivity is localized to Nucleus significantly.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
