Application
HOOK3 Antibody (Center) western blot analysis in 293,HepG2 cell line lysates (35ug/lane).This demonstrates the HOOK3 antibody detected the HOOK3 protein (arrow).Application
HOOK3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HOOK3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | HOOK3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HOOK3; Protein Hook homolog 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | HOOK3 Protein Hook homolog 3 |
|---|---|
| Molecular Weight(MW) | 83126 Da |
| Function | Probably serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex) May regulate clearance of endocytosed receptors such as MSR1 Participates in defining the architecture and localization of the Golgi complex |
| Tissue Specificity | This HOOK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 388-418 amino acids from the Central region of human HOOK3. |
| Cellular Localization | Cytoplasm, cytoskeleton. Golgi apparatus. Note=Enriched at the cis-face of the Golgi complex (By similarity). Localizes to microtubule asters in prophase (By similarity) |
| Entrez Gene | 84376 |
|---|---|
| Nucleotide Accession | NP_115786.1 |
Application
HOOK3 Antibody (Center) western blot analysis in 293,HepG2 cell line lysates (35ug/lane).This demonstrates the HOOK3 antibody detected the HOOK3 protein (arrow).
Application
HOOK3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HOOK3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
