Application
Western blot analysis of HP (arrow) using rabbit polyclonal HP Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HP gene.Application
HP antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HP antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
HP Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of HP Antibody (Center)(Cat#AP10680c) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
HP Antibody (Center) western blot analysis in MDA-MB453,CEM,NCI-H460,HepG2,K562 cell line lysates (35ug/lane).This demonstrates the HP antibody detected the HP protein (arrow).| Product Name | HP Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HP; Haptoglobin; Zonulin; Haptoglobin alpha chain; Haptoglobin beta chain |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | HP Haptoglobin Zonulin Haptoglobin alpha chain Haptoglobin beta chain |
|---|---|
| Molecular Weight(MW) | 45205 Da |
| Function | As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway |
| Tissue Specificity | Expressed by the liver and secreted in plasma. |
| Cellular Localization | Secreted. |
| Entrez Gene | 3240 |
|---|---|
| Nucleotide Accession | NP_005134.1 |
Application
Western blot analysis of HP (arrow) using rabbit polyclonal HP Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HP gene.
Application
HP antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HP antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
HP Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of HP Antibody (Center)(Cat#AP10680c) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
HP Antibody (Center) western blot analysis in MDA-MB453,CEM,NCI-H460,HepG2,K562 cell line lysates (35ug/lane).This demonstrates the HP antibody detected the HP protein (arrow).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
