Application
HS3ST5 Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the HS3ST5 antibody detected the HS3ST5 protein (arrow).Application
HS3ST5 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HS3ST5 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | HS3ST5 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HS3ST5; 3OST5; HS3OST5; Heparan sulfate glucosamine 3-O-sulfotransferase 5; Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | 3OST5 HS3OST5 |
|---|---|
| Alternative Names | HS3ST5 3OST5 HS3OST5 Heparan sulfate glucosamine 3-O-sulfotransferase 5 Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 5 |
| Molecular Weight(MW) | 40408 Da |
| Function | Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site. Also generates GlcUA- GlcNS or IdoUA-GlcNS and IdoUA2S-GlcNH2. The substrate-specific O- sulfation generates an enzyme-modified heparan sulfate which acts as a binding receptor to Herpes simplex virus-1 (HSV-1) and permits its entry |
| Tissue Specificity | Highly expressed in skeletal muscle and fetal brain, and also found in adult brain, spinal cord, cerebellum and colon |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein (Probable) |
| Entrez Gene | 222537 |
|---|---|
| Nucleotide Accession | NP_705840.2 |
Application
HS3ST5 Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the HS3ST5 antibody detected the HS3ST5 protein (arrow).
Application
HS3ST5 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HS3ST5 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
