Application
Western blot analysis of HS3ST1 Antibody (N-term) in Jurkat cell line lysates (35ug/lane). HS3ST1 (arrow) was detected using the purified Pab.Application
HS3ST1 Antibody (N-term) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | HS3ST1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HS3ST1; 3OST; 3OST1; Heparan sulfate glucosamine 3-O-sulfotransferase 1; Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | 3OST 3OST1 |
|---|---|
| Alternative Names | HS3ST1 3OST 3OST1 Heparan sulfate glucosamine 3-O-sulfotransferase 1 Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1 |
| Molecular Weight(MW) | 35773 Da |
| Function | Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site |
| Tissue Specificity | Highly expressed in the brain and kidney and weakly expressed in the heart, lung and placenta |
| Cellular Localization | Golgi apparatus lumen (Probable). |
| Entrez Gene | 9957 |
|---|
Application
Western blot analysis of HS3ST1 Antibody (N-term) in Jurkat cell line lysates (35ug/lane). HS3ST1 (arrow) was detected using the purified Pab.
Application
HS3ST1 Antibody (N-term) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
