Application
Western blot analysis of HSD11B2 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). HSD11B2 (arrow) was detected using the purified Pab.Application
HSD11B2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HSD11B2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
HSD11B2 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | HSD11B2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSD11B2; HSD11K; Corticosteroid 11-beta-dehydrogenase isozyme 2; 11-beta-hydroxysteroid dehydrogenase type 2; 11-beta-hydroxysteroid dehydrogenase type II; NAD-dependent 11-beta-hydroxysteroid dehydrogenase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HSD11K |
|---|---|
| Alternative Names | HSD11B2 HSD11K Corticosteroid 11-beta-dehydrogenase isozyme 2 11-beta-hydroxysteroid dehydrogenase type 2 11-beta-hydroxysteroid dehydrogenase type II NAD-dependent 11-beta-hydroxysteroid dehydrogenase |
| Molecular Weight(MW) | 44127 Da |
| Function | Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids |
| Tissue Specificity | Found in placenta, kidney, pancreas, prostate, ovary, small intestine and colon |
| Cellular Localization | Microsome. Endoplasmic reticulum. |
| Entrez Gene | 3291 |
|---|
Application
Western blot analysis of HSD11B2 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). HSD11B2 (arrow) was detected using the purified Pab.
Application
HSD11B2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HSD11B2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
HSD11B2 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
