Application
Western blot analysis of HS3ST2 Antibody (C-term) in Hela, 293 cell line lysates (35ug/lane). HS3ST2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with HS3ST2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
HS3ST2 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | HS3ST2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HS3ST2; 3OST2; Heparan sulfate glucosamine 3-O-sulfotransferase 2; Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | 3OST2 |
|---|---|
| Alternative Names | HS3ST2 3OST2 Heparan sulfate glucosamine 3-O-sulfotransferase 2 Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 2 |
| Molecular Weight(MW) | 41501 Da |
| Function | Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to an N- unsubstituted glucosamine linked to a 2-O-sulfo iduronic acid unit on heparan sulfate. Catalyzes the O-sulfation of glucosamine in GlcA2S-GlcNS. Unlike 3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate |
| Tissue Specificity | Highly expressed in the brain and weakly expressed in the heart, placenta, lung and skeletal muscle |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein (Probable) |
| Entrez Gene | 9956 |
|---|
Application
Western blot analysis of HS3ST2 Antibody (C-term) in Hela, 293 cell line lysates (35ug/lane). HS3ST2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with HS3ST2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
HS3ST2 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
