Application
Western blot analysis of HSD17B2 Antibody (Center) in MDA-MB435 cell line lysates (35ug/lane).HSD17B2 (arrow) was detected using the purified Pab.Application
HSD17B2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HSD17B2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
HSD17B2 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | HSD17B2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSD17B2; EDH17B2; Estradiol 17-beta-dehydrogenase 2; 17-beta-hydroxysteroid dehydrogenase type 2; 20 alpha-hydroxysteroid dehydrogenase; E2DH; Microsomal 17-beta-hydroxysteroid dehydrogenase; Testosterone 17-beta-dehydrogenase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EDH17B2 |
|---|---|
| Alternative Names | HSD17B2 EDH17B2 Estradiol 17-beta-dehydrogenase 2 17-beta-hydroxysteroid dehydrogenase type 2 20 alpha-hydroxysteroid dehydrogenase E2DH Microsomal 17-beta-hydroxysteroid dehydrogenase Testosterone 17-beta-dehydrogenase |
| Molecular Weight(MW) | 42785 Da |
| Function | Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH |
| Tissue Specificity | This HSD17B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-300 amino acids from the Central region of human HSD17B2. |
| Cellular Localization | Membrane; Single-pass type II membrane protein (Potential) |
| Entrez Gene | 3294 |
|---|
Application
Western blot analysis of HSD17B2 Antibody (Center) in MDA-MB435 cell line lysates (35ug/lane).HSD17B2 (arrow) was detected using the purified Pab.
Application
HSD17B2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HSD17B2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
HSD17B2 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
