Application
Western blot analysis of HSD17B6 Antibody (N-term) in CEM cell line lysates (35ug/lane). HSD17B6 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with HSD17B6 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | HSD17B6 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSD17B6; RODH; 17-beta-hydroxysteroid dehydrogenase type 6; 3-alpha->beta-hydroxysteroid epimerase; Oxidative 3-alpha hydroxysteroid dehydrogenase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RODH |
|---|---|
| Alternative Names | HSD17B6 RODH 17-beta-hydroxysteroid dehydrogenase type 6 3-alpha->beta-hydroxysteroid epimerase Oxidative 3-alpha hydroxysteroid dehydrogenase |
| Molecular Weight(MW) | 35966 Da |
| Function | NAD-dependent oxidoreductase with broad substrate specificity that shows both oxidative and reductive activity (in vitro). Has 17-beta-hydroxysteroid dehydrogenase activity towards various steroids (in vitro). Converts 5-alpha-androstan-3- alpha,17-beta-diol to androsterone and estradiol to estrone (in vitro). Has 3-alpha-hydroxysteroid dehydrogenase activity towards androsterone (in vitro). Has retinol dehydrogenase activity towards all-trans-retinol (in vitro). Can convert androsterone to epi-androsterone. Androsterone is first oxidized to 5-alpha- androstane-3,17-dione and then reduced to epi-andosterone. Can act on both C-19 and C-21 3-alpha-hydroxysteroids |
| Tissue Specificity | Detected in liver and prostate (at protein level). Detected in adult liver, lung, brain, placenta, prostate, adrenal gland, testis, mammary gland, spleen, spinal cord and uterus. Detected in caudate nucleus, and at lower levels in amygdala, corpus callosum, hippocampus, substantia nigra and thalamus. Detected in fetal lung, liver and brain |
| Cellular Localization | Microsome membrane; Peripheral membrane protein; Lumenal side. Early endosome membrane; Peripheral membrane protein; Lumenal side (Potential) |
| Entrez Gene | 8630 |
|---|
Application
Western blot analysis of HSD17B6 Antibody (N-term) in CEM cell line lysates (35ug/lane). HSD17B6 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with HSD17B6 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
