Application
HSD3B1 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the HSD3B1 antibody detected the HSD3B1 protein (arrow).Application
Western blot analysis of HSD3B1 (arrow) using rabbit polyclonal HSD3B1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HSD3B1 gene.| Product Name | HSD3B1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSD3B1; 3BH; HSDB3A; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I; Trophoblast antigen FDO161G; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-hydroxy-5-ene steroi |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | 3BH HSDB3A |
|---|---|
| Alternative Names | HSD3B1 3BH HSDB3A 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I Trophoblast antigen FDO161G 3-beta-hydroxy-Delta(5)-steroid dehydrogenase 3-beta-hydroxy-5-ene steroi |
| Molecular Weight(MW) | 42252 Da |
| Function | 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17- alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone |
| Tissue Specificity | Placenta and skin. Predominantly expressed in mammary gland tissue |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein |
| Entrez Gene | 3283 |
|---|---|
| Nucleotide Accession | NP_000853.1 |
Application
HSD3B1 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the HSD3B1 antibody detected the HSD3B1 protein (arrow).
Application
Western blot analysis of HSD3B1 (arrow) using rabbit polyclonal HSD3B1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HSD3B1 gene.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
