Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-HSF1 (pS320) RabMAb (Cat. #AJ1374c), 1:20,000 dilution. Cells were either (A) untreated (B) treated with heat (42°C)Application
B. IHC Analysis using anti-Phospho-HSF1 (pS320) RabMAb (Cat. #AJ1374c),| Product Name | HSF1 Antibody Phospho (pS320) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSF1, HSTF1, Heat shock factor protein 1, Heat shock transcription factor 1 |
| Modification | p-S320 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR1712 |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:10000~20000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HSTF1 |
|---|---|
| Alternative Names | HSF1 HSTF1 Heat shock factor protein 1 Heat shock transcription factor 1 |
| Molecular Weight(MW) | 57260 Da |
| Function | DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked |
| Tissue Specificity | A phospho specific peptide corresponding to residues surrounding serine 320 of human HSF1 was used as an immunogen. This antibody detects HSF1 phosphorlyated on serine 320. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules |
| Entrez Gene | 3297 |
|---|
Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-HSF1 (pS320) RabMAb (Cat. #AJ1374c), 1:20,000 dilution. Cells were either (A) untreated (B) treated with heat (42°C)
Application
B. IHC Analysis using anti-Phospho-HSF1 (pS320) RabMAb (Cat. #AJ1374c),| Application Notes | WB:1:10000~20000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
