Application
Western blot analysis of HSF1 polyclonal antibody (Cat# AP2501a) in 293 cell line lysates. Non-sumoylated HSF1 (arrow) was detected using the purified Pab.Application
Western blot analysis of HSF1 (arrow) using rabbit polyclonal HSF1 Antibody (Sumo) (Cat# AP2501a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HSF1 gene.Application
Confocal immunofluorescent analysis of HSF1 Sumoylation Site Antibody (Cat#AP2501a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | HSF1 Sumoylation Site Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSF1; HSTF1; Heat shock factor protein 1; Heat shock transcription factor 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HSTF1 |
|---|---|
| Alternative Names | HSF1 HSTF1 Heat shock factor protein 1 Heat shock transcription factor 1 |
| Molecular Weight(MW) | 57260 Da |
| Function | DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked |
| Tissue Specificity | This HSF1 Sumoylation Site antibody is generated from rabbits immunized with the KLH conjugated synthetic peptide PLVRVKEEPPSPPQSPR containing a predicted sumoylation site from the central region of human HSF1. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules |
| Entrez Gene | 3297 |
|---|
Application
Western blot analysis of HSF1 polyclonal antibody (Cat# AP2501a) in 293 cell line lysates. Non-sumoylated HSF1 (arrow) was detected using the purified Pab.
Application
Western blot analysis of HSF1 (arrow) using rabbit polyclonal HSF1 Antibody (Sumo) (Cat# AP2501a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HSF1 gene.
Application
Confocal immunofluorescent analysis of HSF1 Sumoylation Site Antibody (Cat#AP2501a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
