Application
A. Western blot analysis on Human skeletal muscle lysates using anti-Phospho-HSL (pS855) RabMAb (Cat. #AJ1377a), 1:5000 dilution. Cells were either (A) untreated (B) treated with LP| Product Name | HSL Antibody Phospho (pS855) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LIPE, Hormone-sensitive lipase |
| Modification | p-S855 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2330 |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000~5000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | LIPE Hormone-sensitive lipase |
|---|---|
| Molecular Weight(MW) | 116598 Da |
| Function | In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production |
| Tissue Specificity | A phospho-specific peptide corresponding to residues surrounding Serine 855 of human HSL was used as an immunogen. The antibody only detects HSL phosphorylated on Serine 855. |
| Cellular Localization | Cell membrane. Membrane, caveola. Cytoplasm, cytosol. Note=Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation |
| Entrez Gene | 3991 |
|---|
Application
A. Western blot analysis on Human skeletal muscle lysates using anti-Phospho-HSL (pS855) RabMAb (Cat. #AJ1377a), 1:5000 dilution. Cells were either (A) untreated (B) treated with LP| Application Notes | WB:1:1000~5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
