Application
Western blot analysis of anti-HSP40 Antibody in Hela cell line lysates (35ug/lane). HSP40 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with HSP40 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | HSP40 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNAJB1; DNAJ1; HDJ1; HSPF1; DnaJ homolog subfamily B member 1; DnaJ protein homolog 1; Heat shock 40 kDa protein 1; Human DnaJ protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DNAJ1 HDJ1 HSPF1 |
|---|---|
| Alternative Names | DNAJB1 DNAJ1 HDJ1 HSPF1 DnaJ homolog subfamily B member 1 DnaJ protein homolog 1 Heat shock 40 kDa protein 1 Human DnaJ protein 1 |
| Molecular Weight(MW) | 38044 Da |
| Function | Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP |
| Tissue Specificity | This HSP40 antibody is generated from rabbits immunized with a recombinant protein encoding full length of human HSP40. |
| Cellular Localization | Cytoplasm. Nucleus. Nucleus, nucleolus. Note=Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock |
Application
Western blot analysis of anti-HSP40 Antibody in Hela cell line lysates (35ug/lane). HSP40 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with HSP40 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
