Application
Western blot analysis of HSP90AB1 Antibody (Center) in T47D, A2058 and A375 cell line lysates (35ug/lane). HSP90AB1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with HSP90AB1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of WiDr cells using HSP90AB1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | HSP90AB1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSP90AB1; HSP90B; HSPC2; HSPCB; Heat shock protein HSP 90-beta; Heat shock 84 kDa |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HSP90B HSPC2 HSPCB |
|---|---|
| Alternative Names | HSP90AB1 HSP90B HSPC2 HSPCB Heat shock protein HSP 90-beta Heat shock 84 kDa |
| Molecular Weight(MW) | 83264 Da |
| Function | Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function |
| Tissue Specificity | This HSP90AB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444~474 amino acids from the Center region of human HSP90AB1. |
| Cellular Localization | Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV |
| Entrez Gene | 3326 |
|---|
Application
Western blot analysis of HSP90AB1 Antibody (Center) in T47D, A2058 and A375 cell line lysates (35ug/lane). HSP90AB1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with HSP90AB1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of WiDr cells using HSP90AB1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
