Application
A. Western blot analysis on (A) Jurkat, (B) 293T and (C) HeLa cell lysates using anti-HtrA2 RabMAb (Cat. #AJ1384a), dilution 1:5000.Application
B. Immunohistochemical analysis of paraffin-embedded human colon using anti-HtrA2 RabMAb (Cat. #AJ1384a).| Product Name | HtrA2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HTRA2, OMI, PRSS25, Serine protease HTRA2, mitochondrial, Omi stress-regulated endoprotease;Serine proteinase OMI;Serine protease 25 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR22 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:20000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | OMI PRSS25 |
|---|---|
| Alternative Names | HTRA2 OMI PRSS25 Serine protease HTRA2 mitochondrial Omi stress-regulated endoprotease Serine proteinase OMI Serine protease 25 |
| Molecular Weight(MW) | 48841 Da |
| Function | Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive |
| Tissue Specificity | Isoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid |
| Cellular Localization | Mitochondrion intermembrane space. Mitochondrion membrane; Single-pass membrane protein (Potential) Note=Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment, and stimulation of mitochondria with caspase-8 truncated BID/tBID |
| Entrez Gene | 27429 |
|---|
Application
A. Western blot analysis on (A) Jurkat, (B) 293T and (C) HeLa cell lysates using anti-HtrA2 RabMAb (Cat. #AJ1384a), dilution 1:5000.
Application
B. Immunohistochemical analysis of paraffin-embedded human colon using anti-HtrA2 RabMAb (Cat. #AJ1384a).| Application Notes | WB:1:20000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
