Application
HTRA2 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HTRA2 antibody detected the HTRA2 protein (arrow).Application
Western blot analysis of HTRA2 (arrow) using rabbit polyclonal HTRA2 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HTRA2 gene.Application
HTRA2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HTRA2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | HTRA2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HTRA2; OMI; PRSS25; Serine protease HTRA2, mitochondrial; High temperature requirement protein A2; Omi stress-regulated endoprotease; Serine protease 25; Serine proteinase OMI |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | OMI PRSS25 |
|---|---|
| Alternative Names | HTRA2 OMI PRSS25 Serine protease HTRA2 mitochondrial High temperature requirement protein A2 Omi stress-regulated endoprotease Serine protease 25 Serine proteinase OMI |
| Molecular Weight(MW) | 48841 Da |
| Function | Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive |
| Tissue Specificity | Isoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid |
| Cellular Localization | Mitochondrion intermembrane space. Mitochondrion membrane; Single-pass membrane protein (Potential) Note=Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment, and stimulation of mitochondria with caspase-8 truncated BID/tBID |
| Entrez Gene | 27429 |
|---|---|
| Nucleotide Accession | NP_659540.1 NP_037379.1 |
Application
HTRA2 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HTRA2 antibody detected the HTRA2 protein (arrow).
Application
Western blot analysis of HTRA2 (arrow) using rabbit polyclonal HTRA2 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HTRA2 gene.
Application
HTRA2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HTRA2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
