Application
Western blot analysis of Hus1 (arrow) using rabbit polyclonal hHus1 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Hus1 gene (Lane 2) (Origene Technologies).| Product Name | Hus1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HUS1; Checkpoint protein HUS1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | HUS1 Checkpoint protein HUS1 |
|---|---|
| Molecular Weight(MW) | 31691 Da |
| Function | Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Nucleus. Cytoplasm. Note=In discrete nuclear foci upon DNA damage. According to PubMed:14500360, localized also in the cytoplasm. DNA damage induces its nuclear translocation Shuttles between the nucleus and the cytoplasm |
| Entrez Gene | 3364 |
|---|
Application
Western blot analysis of Hus1 (arrow) using rabbit polyclonal hHus1 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Hus1 gene (Lane 2) (Origene Technologies).| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
