Application
IBTK Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the IBTK antibody detected the IBTK protein (arrow).Application
IBTK antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IBTK antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
IBTK Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | IBTK Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IBTK; BTKI; KIAA1417; Inhibitor of Bruton tyrosine kinase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BTKI KIAA1417 |
|---|---|
| Alternative Names | IBTK BTKI KIAA1417 Inhibitor of Bruton tyrosine kinase |
| Molecular Weight(MW) | 150528 Da |
| Function | Acts as an inhibitor of BTK tyrosine kinase activity, thereby playing a role in B-cell development. Down-regulates BTK kinase activity, leading to interference with BTK-mediated calcium mobilization and NF-kappa-B-driven transcription |
| Tissue Specificity | Expressed in DeFew, HEK293T, HeLa and in Jurkat, MC3 and NB4 lymphoid cells (at protein level). Isoform 1 is the predominant isoform expressed in all examined tissues and cell lines. Highly expressed in hemopoietic tissues (fetal liver, spleen, lymph node, thymus, peripheral blood leukocytes and bone marrow). Weakly or not expressed in other tissues |
| Cellular Localization | Cytoplasm. Membrane; Peripheral membrane protein. Note=Translocates to the plasma membrane upon IgM stimulation |
| Entrez Gene | 25998 |
|---|---|
| Nucleotide Accession | NP_056340.2 |
Application
IBTK Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the IBTK antibody detected the IBTK protein (arrow).
Application
IBTK antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IBTK antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
IBTK Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
