Application
Western blot analysis of ID4 Antibody (N-term) in mouse bladder tissue lysates (35ug/lane). ID4 (arrow) was detected using the purified Pab.Application
ID4 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ID4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
ID4 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ID4 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ID4; BHLHB27; DNA-binding protein inhibitor ID-4; Class B basic helix-loop-helix protein 27; Inhibitor of DNA binding 4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BHLHB27 |
|---|---|
| Alternative Names | ID4 BHLHB27 DNA-binding protein inhibitor ID-4 Class B basic helix-loop-helix protein 27 Inhibitor of DNA binding 4 |
| Molecular Weight(MW) | 16622 Da |
| Function | ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding |
| Tissue Specificity | This ID4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human ID4. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 3400 |
|---|
Application
Western blot analysis of ID4 Antibody (N-term) in mouse bladder tissue lysates (35ug/lane). ID4 (arrow) was detected using the purified Pab.
Application
ID4 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ID4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
ID4 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
