Application
Western blot analysis of anti-IDE Antibody (Center) in A375 cell line lysates (35ug/lane). IDE (arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDE antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | IDE Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IDE; Insulin-degrading enzyme; Abeta-degrading protease; Insulin protease; Insulysin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | IDE Insulin-degrading enzyme Abeta-degrading protease Insulin protease Insulysin |
|---|---|
| Molecular Weight(MW) | 117968 Da |
| Function | Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia |
| Tissue Specificity | This IDE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 413~443 amino acids from the Center region of human IDE. |
| Cellular Localization | Cytoplasm. Cell membrane. Secreted (By similarity). Note=Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform |
| Entrez Gene | 3416 |
|---|
Application
Western blot analysis of anti-IDE Antibody (Center) in A375 cell line lysates (35ug/lane). IDE (arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDE antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
