Application
IER3 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the IER3 antibody detected the IER3 protein (arrow).Application
IER3 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the IER3 antibody detected the IER3 protein (arrow).Application
IER3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IER3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of IER3 Antibody (N-term)(Cat#AP11790a) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
IER3 Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | IER3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IER3; DIF2; IEX1; PRG1; Radiation-inducible immediate-early gene IEX-1; Differentiation-dependent gene 2 protein; Immediate early protein GLY96; Immediate early response 3 protein; PACAP-responsive gene 1 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DIF2 IEX1 PRG1 |
|---|---|
| Alternative Names | IER3 DIF2 IEX1 PRG1 Radiation-inducible immediate-early gene IEX-1 Differentiation-dependent gene 2 protein Immediate early protein GLY96 Immediate early response 3 protein PACAP-responsive gene 1 protein |
| Molecular Weight(MW) | 16903 Da |
| Function | May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A- PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment |
| Tissue Specificity | This IER3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-73 amino acids from the N-terminal region of human IER3. |
| Cellular Localization | Membrane; Single-pass type II membrane protein |
| Entrez Gene | 8870 |
|---|---|
| Nucleotide Accession | NP_003888.2 |
Application
IER3 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the IER3 antibody detected the IER3 protein (arrow).
Application
IER3 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the IER3 antibody detected the IER3 protein (arrow).
Application
IER3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IER3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of IER3 Antibody (N-term)(Cat#AP11790a) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
IER3 Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
