Application
Confocal immunofluorescent analysis of IDH1 Antibody (Center)(Cat#AP7454c) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDH1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of IDH1 Antibody (Center) in HepG2 cell line and mouse liver tissue lysates (35ug/lane). IDH1 (arrow) was detected using the purified Pab.Application
IDH1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | IDH1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IDH1; PICD; Isocitrate dehydrogenase [NADP] cytoplasmic; Cytosolic NADP-isocitrate dehydrogenase; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHCWBFC |
| IF:1:10~50 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PICD |
|---|---|
| Alternative Names | IDH1 PICD Isocitrate dehydrogenase [NADP] cytoplasmic Cytosolic NADP-isocitrate dehydrogenase IDP NADP(+)-specific ICDH Oxalosuccinate decarboxylase |
| Molecular Weight(MW) | 46659 Da |
| Tissue Specificity | This IDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122~152 amino acids from the Center region of human IDH1. |
| Cellular Localization | Cytoplasm. Peroxisome. |
| Entrez Gene | 3417 |
|---|
Application
Confocal immunofluorescent analysis of IDH1 Antibody (Center)(Cat#AP7454c) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDH1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of IDH1 Antibody (Center) in HepG2 cell line and mouse liver tissue lysates (35ug/lane). IDH1 (arrow) was detected using the purified Pab.
Application
IDH1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | IF:1:10~50 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
