Application
IFIH1 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the IFIH1 antibody detected the IFIH1 protein (arrow).| Product Name | IFIH1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IFIH1; MDA5; RH116; Interferon-induced helicase C domain-containing protein 1; Clinically amyopathic dermatomyositis autoantigen 140 kDa; Helicase with 2 CARD domains; Interferon-induced with helicase C domain protein 1; Melanoma differentiation-associated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MDA5 RH116 |
|---|---|
| Alternative Names | IFIH1 MDA5 RH116 Interferon-induced helicase C domain-containing protein 1 Clinically amyopathic dermatomyositis autoantigen 140 kDa Helicase with 2 CARD domains Interferon-induced with helicase C domain protein 1 Melanoma differentiation-associated |
| Molecular Weight(MW) | 116689 Da |
| Function | Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2'-O- methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV) and mengo encephalomyocarditis virus (ENMG). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines |
| Tissue Specificity | Widely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung |
| Cellular Localization | Cytoplasm. Nucleus (Potential). Note=May be found in the nucleus, during apoptosis |
| Entrez Gene | 64135 |
|---|---|
| Nucleotide Accession | NP_071451.2 |
Application
IFIH1 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the IFIH1 antibody detected the IFIH1 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
