Application
IFT57 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the IFT57 antibody detected the IFT57 protein (arrow).Application
IFT57 Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the IFT57 antibody detected the IFT57 protein (arrow).Application
IFT57 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IFT57 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
IFT57 Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | IFT57 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IFT57; DERP8; ESRRBL1; HIPPI; Intraflagellar transport protein 57 homolog; Dermal papilla-derived protein 8; Estrogen-related receptor beta-like protein 1; HIP1-interacting protein; MHS4R2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DERP8 ESRRBL1 HIPPI |
|---|---|
| Alternative Names | IFT57 DERP8 ESRRBL1 HIPPI Intraflagellar transport protein 57 homolog Dermal papilla-derived protein 8 Estrogen-related receptor beta-like protein 1 HIP1-interacting protein MHS4R2 |
| Molecular Weight(MW) | 49108 Da |
| Function | Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro- apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear |
| Tissue Specificity | Present in many tissues such as brain, thymus, lymph node, lung, liver, skin and kidney (at protein level) |
| Cellular Localization | Cytoplasm, cytoskeleton, cilium basal body (By similarity). Note=Concentrates within the inner segment of cilia (By similarity) |
| Entrez Gene | 55081 |
|---|---|
| Nucleotide Accession | NP_060480.1 |
Application
IFT57 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the IFT57 antibody detected the IFT57 protein (arrow).
Application
IFT57 Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the IFT57 antibody detected the IFT57 protein (arrow).
Application
IFT57 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IFT57 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
IFT57 Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
