Application
Anti-IFT57 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with IFT57Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFT57 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFT57.| Product Name | HIPPI antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1H2] to HIPPIRequired for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator | however the relevance of such function remains unclear. |
| Immunogen | Full length human recombinant protein of human HIPPI ( NP_060480) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1H2 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| ICC/IF 1:100,: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ESRRBL1 MHS4R2 IFT57 FLJ10147 HIPPI |
|---|---|
| Target | HIPPI |
| Tissue Specificity | is specific for human HIPPI ( NP_060480). |
| Cellular Localization | Concentrates within the inner segment of cilia. |
Application
Anti-IFT57 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with IFT57
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFT57 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFT57.| Application Notes | ICC/IF 1:100,: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
