Application
Western blot analysis of IGJ antibody (N-term) (Cat.# AP6548a) in mouse spleen lysates (35ug/lane). IGJ (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded mouse spleen reacted with IGJ Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
IGJ Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | IGJ Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IGJ; IGCJ; Immunoglobulin J chain |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IGCJ |
|---|---|
| Alternative Names | IGJ IGCJ Immunoglobulin J chain |
| Molecular Weight(MW) | 18099 Da |
| Function | Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also help to bind these immunoglobulins to secretory component |
| Tissue Specificity | This IGJ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17~47 amino acids from the N-terminal region of human IGJ. |
| Cellular Localization | Secreted. |
| Entrez Gene | 3512 |
|---|
Application
Western blot analysis of IGJ antibody (N-term) (Cat.# AP6548a) in mouse spleen lysates (35ug/lane). IGJ (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded mouse spleen reacted with IGJ Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
IGJ Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
