Application
IL12B antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL12B antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
IL12B Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
IL12B Antibody (C-term) western blot analysis in CEM,MDA-MB453 cell line lysates (35ug/lane).This demonstrates the IL12B antibody detected the IL12B protein (arrow).| Product Name | IL12B Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL12B; NKSF2; Interleukin-12 subunit beta; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL-12 subunit p40; NK cell stimulatory factor chain 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCFCWB |
| IHC:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NKSF2 |
|---|---|
| Alternative Names | IL12B NKSF2 Interleukin-12 subunit beta Cytotoxic lymphocyte maturation factor 40 kDa subunit IL-12 subunit p40 NK cell stimulatory factor chain 2 |
| Molecular Weight(MW) | 37169 Da |
| Function | Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated killer cells, and stimulate the production of IFN-gamma by resting PBMC |
| Tissue Specificity | This IL12B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-298 amino acids from the C-terminal region of human IL12B. |
| Cellular Localization | Secreted. |
| Entrez Gene | 3593 |
|---|---|
| Nucleotide Accession | NP_002178.2 |
Application
IL12B antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL12B antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
IL12B Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
IL12B Antibody (C-term) western blot analysis in CEM,MDA-MB453 cell line lysates (35ug/lane).This demonstrates the IL12B antibody detected the IL12B protein (arrow).| Application Notes | IHC:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
