Application
Western blot analysis of IL12A Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). IL12A (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with IL12A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
IL12A Antibody (C-term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | IL12A Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL12A; NKSF1; Interleukin-12 subunit alpha; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; NK cell stimulatory factor chain 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NKSF1 |
|---|---|
| Alternative Names | IL12A NKSF1 Interleukin-12 subunit alpha Cytotoxic lymphocyte maturation factor 35 kDa subunit IL-12 subunit p35 NK cell stimulatory factor chain 1 |
| Molecular Weight(MW) | 24874 Da |
| Function | Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC |
| Tissue Specificity | This IL12A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174~204 amino acids from the C-terminal region of human IL12A. |
| Cellular Localization | Secreted. |
| Entrez Gene | 3592 |
|---|
Application
Western blot analysis of IL12A Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). IL12A (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with IL12A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
IL12A Antibody (C-term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
