Western blot analysis of IL1A (arrow) using rabbit polyclonal IL1A-pS87 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the IL1A gene (Lane 2) (Origene Technologies).
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells
Tissue Specificity
This IL1A-pS87 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the amino acid residues surrounding S87 of human IL1A.
Cellular Localization
Secreted. Note=The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins
Western blot analysis of IL1A (arrow) using rabbit polyclonal IL1A-pS87 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the IL1A gene (Lane 2) (Origene Technologies).
Application Notes
WB:1:100~500
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.